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NOG-IL15

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NOG-IL15

Next Generation Severely Immunodeficient NOG-IL15 mouse

Next Generation Severely Immunodeficient NOG-IL15 mouse
Strain Name NOG-hIL-15 Tg (NOG-IL15)
  • You will be required to sign a consent form upon purchase.
  • The use of this mouse strain corresponds to the use of Living Modified Organism (Cartagene Protocol, domestic law). We will send you an information provision form in advance, so please check it and use it according to the rules of each institution.
Development

A vector containing the human IL-5 gene downstream of the CMV promoter was microinjected into (NOD♀ x NOG♂) F1 pronuclear stage embryos, and crossed with NOG mouse to establish them.

Product Features
  • This is a severely immunodeficient NOG mouse that expresses hIL-15.
  • When human hematopoietic stem cells are engrafted, differentiation into NK cells is dominant compared to NOG mouse.
  • Differentiated NK cells express various NK receptors and produce granzyme A and perforin.
  • By engrafting PBMC derived CD56 positive NK cells into NOG-IL15 mouse, human NK cells can be engrafted and expanded.
  • Suitable for studying Antibody Dependent Cellular Cytotoxicity (ADCC) activity.
Research Applications
  • Useful as a human cancer immune model with human NK cells as effectors.
  • In vivo model of human NK cells Research on anti-tumor effect by ADCC, NK cytotoxicity

Humanized NOG-IL15 mouse

  • A model engrafted with human peripheral blood NK cells is commonly used.
  • Normally, we do not carry out contract production.
Product features of human NK cells transferred humanized NOG-IL15 mouse
  • Engraftment of human NK cells purified from human peripheral blood into NOG-IL15 mouse resulted in NK cell proliferation that persisted for more than 3 months without signs of GVHD (xenograft-host reaction).
  • NK cells expanded in vivo maintain the original expression pattern of various surface antigens, including NK receptors and killer cell immunoglobulin-like receptor (KIR) molecules, and produce significant amounts of granzyme A and perforin.
  • Transplantation of K562 leukemia cells into NOG-IL15 mouse engrafted with human peripheral blood NK cells resulted in significant suppression of tumor growth compared with non-engrafted mouse with human NK cells.
  • Furthermore, NOG-IL15 mouse allowed engraftment of in vitro expanded NK cells prepared for clinical cell therapy. These cells exhibited antibody-dependent cellular cytotoxicity (ADCC) activity on Her2 positive gastric cancer cells in the presence of therapeutic anti-Her2 antibodies, suppressing tumor growth.
  • Our results suggest that NOG-IL15 mouse is a useful model to study human NK cell biology and assess human NK cell mediated in vivo cytotoxicity.
    (Katano et al. Sci. rep 2017)
  • We are providing detailed by online regarding the characteristics of humanized mouse engrafted with human NK cells, the production of humanized mouse, and experimental protocols for use. Please feel free to contact us at sales@invivoscience.com.
Background data

References

Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouse. (Original, NOG-IL15, NK cell, ADCC, CTL)
Sci Rep. 2017 Dec 8;7(1):17230. doi: 10.1038/s41598-017-17442-7.
Katano I, Nishime C, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M, Takahashi T.

Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease.(humanized mice, NOG-IL15, NK cells)
Methods Mol Biol. 2022;2463:53-66. doi: 10.1007/978-1-0716-2160-8_5.
Shan L, Flavell RA, Herndler-Brandstetter D.

Ruxolitinib, a JAK1/JAK2 selective inhibitor, ameliorates acute and chronic steroid-refractory GvHD mouse models. (humanized mice, NOG-IL15, NK cell, GVHD)
Immunotherapy. 2021 Aug;13(12):977-987. doi: 10.2217/imt-2021-0013. Epub 2021 Jun 29.
Huarte E, Peel M, Juvekar A, Dubé P, Sarah S, Stephens L, Stewart B, Long B, Czerniak P, Oliver J, Smith P.

Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. (NOG-IL15,NK cell mediated ADCC)
Front Immunol. 2020 Oct 7;11:532684. doi: 10.3389/fimmu.2020.532684. eCollection 2020.
Katano I, Ito R, Kawai K, Takahashi T.