Strain Name	NOG-hGM-CSF/hIL-3 Tg (NOG-EXL)

• You will be required to sign a consent form upon purchase.
• The use of this mouse strain corresponds to the use of Living Modified Organism (Cartagene Protocol, domestic law). We will send you an information provision form in advance, so please check it and use it according to the rules of each institution.

Development

NOG-EXL mouse was established the following method. Vectors containing human IL-3 or GM-CSF genes downstream of the SRa promoter were microinjected into C57BL/6J-scid mouse embryos and the resulting transgenic mouse was backcrossed to NOG mouse.

Product features of NOG-EXL mouse

The followings are added to the features of the NOG mouse.

• Increased differentiation of myeloid lineage cells and CD4+ T cells:
  Increased differentiation of myeloid cells such as macrophages, dendritic cells, granulocytes, and mast cells, and CD4+ T cells has been confirmed after human hematopoietic stem cell engraftment.
  It is now possible to conduct research targeting myeloid immune cells, which had been difficult to evaluate using conventional NOG mouse.
• Useful for research on anti-tumor human plasmacytoid dendritic cells (pDC).
  In September 2020, a paper was published stating that NOG-EXL mouse is useful as a model for studying the functions of in vivo tumor-promoting and anti-tumor human plasmacytoid dendritic cells (pDC).
  Ilona et al., Front. Immunol., 2020

Research Applications

• Human hematopoietic and immunology related research models, especially differentiation of myeloid cells, evaluation experiments for anti-tumor effects, etc.
• Human myeloid leukemia tumor-bearing model (AML, CML etc.)
• Analysis of human innate immune responses
• In vivo evaluation model for human mast cell-mediated allergic reactions
• Bone marrow toxicity evaluation experiment for anticancer drugs

Background data

• NOG-EXL Bodyweight change
• NOG-EXL Hematology data
• NOG-EXL Organ weight data

• Human hematopoietic stem cell (HSC) engrafted models are commonly used.
• We produce humanized NOG-EXL mouse in Japan and deliver them to our customers.
• Production will be started after receiving your firm order. We usually ship about 12 weeks after an order is placed.
• You will be required to sign a consent form upon purchase.
• The use of this mouse strain corresponds to the use of Living Modified Organism (Cartagene Protocol, domestic law). We will send you an information provision form in advance, so please check it and use it according to the rules of each institution.

Product features of humanized NOG-EXL mouse engrafted with Hematopoietic Stem Cells (HSC)

• When HSCs are engrafted, myeloid human cells differentiate first. They later differentiate into human T cells and human B cells and engraft.
• Unlike conventional NOG mouse, enhanced differentiation of myeloid cells such as macrophages, dendritic cells, granulocytes, mast cells and CD4 positive T cells has been confirmed. Differentiation into NK cells is minimal.
• Humanized mouse has been confirmed to survive for more than 6 months after engraftment. It has been confirmed that the engraftment ratio of human immune cells remains almost unchanged up to 20 weeks after HSCs engraftment.
• Graft versus host disease (GVHD) caused by human immune cells is not usually seen. Even if cancer cells are transplanted after engraftment of human immune cells, it has been confirmed that engraftment of cancer cells occur in many cases.
• Compared to conventional humanized NOG mouse, the chimeric ratio of human cells is higher, and macrophages may phagocytize mouse erythrocytes and develop anemia.
• In the US and EU countries, TACONIC Biosciences Inc. supplies humanized NOG-EXL mouse. The quality standard is the same as that of humanized NOG-EXL mouse supplied in Japan.

Research Applications

• Human hematopoietic and immunology related research models, especially differentiation of myeloid cells, evaluation experiments for anti-tumor effects, etc.
• Human myeloid leukemia tumor-bearing model (AML, CML etc.).
• Analysis of human innate immune responses.
• In vivo evaluation model for human mast cell-mediated allergic reactions.
• Bone marrow toxicity evaluation experiment for anticancer drugs.
• We are providing detailed briefings online regarding the products features of humanized NOG-EXL mouse, the production of humanized NOG-EXL mouse, and experimental protocols for their use. Please feel free to contact us at sales@invivoscience.com.

Background data

• Human CD45+ positive cell chimeric ratio after transfer of NOG-EXL human cord blood hematopoietic stem cells (CD34+)
• Differentiated cell ratio in humanized NOG-EXL after human Cord Blood Hematopoietic Stem Cell (CD34+) transferred
• NOG-EXL systemic anaphylaxis Model
• Anaphylaxis model by intratracheal administration of IL-33 in NOG-EXL

Video: NOG-EXL and humanized NOG-EXL

We will introduce the development history of NOG-EXL mouse, the characteristics of NOG-EXL mouse engrafted with HSCs (humanized mouse), and their main research applications.